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Podoplanin, PE-Cyanine7, clone: eBio8.1.1 (8.1.1), eBioscience™

Syrian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-5381-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15588556

  • £186.95 / 100µg


EU Stock 3
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Description

Description

Description: The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43kDa transmembrane glycoprotein, named for its expression in kidney glomerular epithelial cells (podocytes). In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum. It was also shown to be a potent marker for lymphatic endothelium. Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation.Applications Reported: This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of the TE-71 cell line. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Podoplanin
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q62011
CTA-520D8.1, AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2
Golden Syrian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
eBio8.1.1 (8.1.1)
PE-Cyanine7
Pdpn
Conjugated
Pdpn
IgG
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
14726
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