Podoplanin, eFluor 660, clone: eBio8.1.1 (8.1.1), eBioscience™
Syrian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 50-5381-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43kDa transmembrane glycoprotein, named for its expression in kidney glomerular epithelial cells (podocytes). In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum. It was also shown to be a potent marker for lymphatic endothelium. Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation.Applications Reported: This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of the TE-71 cell line. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|CTA-520D8.1, AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.