Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), eFluor™ 660, eBioscience™, Invitrogen™

Syrian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-5381-82

Product Code. 15351920

Description

Description: The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein, named for its expression in kidney glomerular epithelial cells (podocytes). In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum. It was also shown to be a potent marker for lymphatic endothelium. Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation. Applications Reported: This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of the TE-71 cell line. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm).

Podoplanin Monoclonal antibody specifically detects Podoplanin in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: Podoplanin
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q62011
• Gene Symbols: PDPN
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14726
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBio8.1.1 (8.1.1)
• Conjugate: eFluor 660
• Gene: PDPN
• Gene Alias: 29kDa cytosolic podoplanin intracellular domain; Aggrus; CTA-520D8.1; E11; E11 antigen epitope; epithelial cell surface transmembrane protein antigen; glycoprotein 36; Glycoprotein 38; glycoprotein, 36-KD; GP36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); Ots8; OTS-8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26; PA2.26 antigen; PDPN; PICD; Podoplanin; PSEC0003; PSEC0025; pulmonary type I alveolar epithelial cell transmembrane differentiation marker; RANDAM-2; RP23-348F1.2; RTI140; RTI40; T1a; T1A2; T1A-2; T1alpha; T1-alpha; TI1A; Transmembrane glycoprotein E11; type I cell 40 kDa protein
• Host Species: Syrian Hamster
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

For Research Use Only.