Podocalyxin, PE, clone: B34D1.3, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-8873-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The monoclonal antibody B34D1.3 recognizes human podocalyxin, a protein expressed on undifferentiated human embryonic stem cells (ES), embryonic carcinoma cells (EC), and embryonic germ cells (EG). Podocalxyin is a heavily glycosylated cell adhesion protein with homology to CD34 and endoglycan. It was first identified on kidney podocytes, and then found to be upregulated in a variety of human cancers such as breast and prostate cancer. Like other stem cell-specific markers, the epitope recognized by the B34D1.3 antibody is lost upon cell differentiation.The B34D1.3 antibody recognizes the ectodomain of podocalyxin, which is a different epitope from the Tra-1-60 and Tra-1-81 monoclonal antibodies. While Tra1-60 has been found to react with a sialidase-sensitive epitope, the epitope recognized by Tra1-81 has not been fully defined.Applications Reported: This B34D1.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This B34D1.3 antibody has been pre-titrated and tested by flow cytometric analysis of the NTERA cell line. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|