Placental Alkaline Phosphatase Monoclonal Antibody (8B6), eFluor™ 660, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15510167	Affymetrix eBioscience 50-9870-82	100 μg
15500167	Affymetrix eBioscience 50-9870-80	25 μg

Description

Description: The monoclonal antibody 8B6 recognizes human Placental Alkaline Phosphatase (PLAP), a 130 (or 130 with 65 kDa) enzyme expressed by placental syncytiotrophoblastoid cells. This enzyme has slightly different enzyme kinetics from other phosphatases. PLAP can be found anchored to the plasma membrane in addition to the cytoplasm. Expression is found in several germ line cancers; ovarian and testicular. Expression is also present in human embryonic stem cells and bone marrow-derived mesenchymal stromal cells. This 8B6 antibody reacts with both the Regan and Nagao isozymes. Applications Reported: This 8B6 antibody has been reported for use in immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (IHC-P) tissue, and ELISA. Applications Tested: This 8B6 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human placenta with trypsin digestion. This antibody can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Placental Alkaline Phosphatase Monoclonal antibody specifically detects Placental Alkaline Phosphatase in Human samples. It is validated for ELISA, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin).

Specifications

• Antigen: Placental Alkaline Phosphatase
• Concentration: 0.2 mg/mL
• Conjugate: eFluor 660
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 250
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: 8B6
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P05187
• Gene Symbols: ALPP
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: ALPP

For Research Use Only.