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Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis

Ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.

Brand:  Thermo Scientific™ 88700

52.00 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 12146490

  • £78.00 / 5ku
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Description

Description

Thermo Scientific™ Pierce Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.

Pierce Universal Nuclease for Cell Lysis is a genetically engineered endonuclease from Serratia marcescens. The enzyme is produced and purified from E. coli and consists of two identical 30-kDa subunits with two critical disulfide bonds. This indiscriminate endonuclease degrades single-stranded, double-stranded, linear and circular DNA and RNA and is effective over a wide range of temperatures and pH. This enzyme has very high specific activity (100-fold greater than DNase I) and has increased thermal stability compared to other nucleases. Pierce Universal Nuclease is ≥99% pure enzyme, is free of any measurable protease activity and is supplied at a concentration of 250 U/μL. Pierce Universal Nuclease for Cell Lysis is identical in performance to Benzonase™ Nuclease by EMD Merck™.

Highlights:

  • Broad spectrum – degrades all forms of DNA and RNA
  • Highest-quality enzyme – nuclease is at least 99% pure, as tested by SDS-PAGE
  • Robust activity – 100-fold greater specific activity than DNase I
  • Versatile – can be used with a wide variety of cell lysis reagents

Recommended for:

Use with B-PER, Y-PER or other commercial or homebrew cell lysis reagents and/or mechanical disruption to reduce viscosity in protein extracts; Remove DNA and RNA from recombinant protein preparations prior to downstream processing

Storage Requirements

Store in a cool, dry, well-ventilated area, protected from direct sunlight

Specifications

Specifications

At least 99% pure enzyme at 250 U/uL in 50% glycerol (v/v), 20 mM NaCl, 2 mM MgCl2, 20 mM Tris, pH 8.0
Cell Lysis Enzyme
Liquid
Enzyme for Cell Lysis
200 mL Lysate
Pierce Universal Nuclease for Cell Lysis
Store in a cool, dry, well-ventilated area, protected from direct sunlight.
Pierce™
Nuclease
5 kU
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For Research Use Only. Not for use in diagnostic procedures.