Thermo Scientific™ Pierce™ TRITC Rhodamine Dyes and Labeling Kits

Label antibodies, proteins and other molecules for fluorescence imaging (ex 546; em 572-615) using these activated derivatives of red-fluorescent rhodamine dye.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 46115

TEXAS RED SULFONYL CHLORIDE 10MG

UNSPSC: 41116012

Code: PN

Additional Details:
Additional Details: CAS Number: 69097-20-7 Weight: 0.80000kg



Product Code. 11819370

Quantity Price
1 £ 184.0 / 10mg
Estimated Shipment date
from Supplier 07-02-2017
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Description and Specification

Specification

Formulation Sulfonyl chloride activated derivative of rhodamine fluorescent dye
Sufficient For Approx. 5 uses to label a total of 10mg of IgG using typical conditions
Description Pierce Texas Red Sulfonyl Chloride
Quantity 10mg
Item Description Texas Red Sulfonyl Chloride,10milligram

Rhodamine dyes are used in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. TRITC, NHS-rhodamine and Texas Red™ Sulfonyl Chloride are reactive towards primary amine groups on proteins, peptides and other biomolecules. Rhodamine dyes are often a mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

  • Label and purify antibody in about one hour Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification and removal of non-reacted dye with excellent protein recovery

Includes:

NHS-Rhodamine Antibody Labeling Kit (Mfr. No. 53031)

NHS-Rhodamine, 3 vials; Borate Buffer, 1mL; Purification Resin, 5mL; Spin Columns, 6 each; Collection Tubes, 12 each

Documentation

Technical documents