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Thermo Scientific™ Pierce™ TRITC Rhodamine Dyes and Labeling Kits

Label antibodies, proteins and other molecules for fluorescence imaging (ex 546; em 572-615) using these activated derivatives of red-fluorescent rhodamine dye.

Brand:  Thermo Scientific™ 46115

Code : PN

Additional Details : CAS Number : 69097-20-7 Weight : 0.80000kg

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Product Code. 11819370

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Description

Description

Rhodamine dyes are used in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. TRITC, NHS-rhodamine and Texas Red™ Sulfonyl Chloride are reactive towards primary amine groups on proteins, peptides and other biomolecules. Rhodamine dyes are often a mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

  • Label and purify antibody in about one hour Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification and removal of non-reacted dye with excellent protein recovery

Includes:

NHS-Rhodamine Antibody Labeling Kit (Mfr. No. 53031)

NHS-Rhodamine, 3 vials; Borate Buffer, 1mL; Purification Resin, 5mL; Spin Columns, 6 each; Collection Tubes, 12 each

Specifications

Specifications

Pierce Texas Red Sulfonyl Chloride
Sulfonyl chloride activated derivative of rhodamine fluorescent dye
Approx. 5 uses to label a total of 10mg of IgG using typical conditions
10mg
Documentation

Technical Documents