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Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Brand:  Thermo Scientific™ 34029

358.15 GBP valid until 2021-04-30
Use promo code "14803" to get your promotional price.



Code : PN

Additional Details : Weight : 1.00000kg

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Product Code. 12617087

  • £388.00 / 1 L


In stock, ready to ship: 25
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Description

Description

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 and 652nm. The color then changes to yellow (measured at 450nm) upon addition of sulfuric or phosphoric acid to stop the reaction. TMB is very sensitive and more quickly oxidized than other HRP substrates, resulting in faster color development. Unlike other commercially available substrates, these products contain no DMF or DMSO. There are three formulations that differ primarily in their sensitivities. Slow TMB is intermediate in sensitivity, ideal for kinetic readings. The sensitivity of the Turbo TMB compares to that of OPD used at approximately 1mg/mL. Ultra TMB-ELISA produces the highest signal-to-noise ratio and sensitivity in the picogram range.

Highlights:

  • Ready-to-use solutions
  • No hydrogen peroxide required
  • No filtering required
  • Noncarcinogenic
  • Various sensitivities to suit any assay

1-Step TMB Substrates

  • Ready-to-use, one-component substrates
  • Do not require hydrogen peroxide
  • Noncarcinogenic
  • Do not contain DMF or DMSO
  • Available in three formulations that vary by sensitivity

TMB Substrate Kit

  • More sensitive than OPD in endpoint ELISAs
  • Easy to use
  • Provides results in seconds

Includes:

TMB Substrate Kit (PI-34021): Peroxidase substrate, 200mL; Peroxide solution, 200mL

Specifications

Specifications

1-Step™
Colorimetric
Storage: Upon receipt store at 4°C, protected from moisture. Product is shipped at ambient temperature.
ELISA Substrate
Chemical Substrate
HRP (Horseradish Peroxidase) Substrate
Pierce 1-Step Ultra TMB-ELISA Substrate Solution
1 L
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Special Offers

Special Offers

  • Big discounts are available on a range of popular products including chemicals, lab essentials, equipment, consumables, life science and safety products.
    : 14803

    2021-04-30

    *Discount will apply to qualifying orders received by Fisher Scientific between 01/03/2021 and 30/04/2021. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

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