Thermo Scientific™ Pierce™ TiO2 Phosphopeptide Enrichment and Clean-up Kit
Desalt and purify phosphorylated peptides for mass spectrometry using these titanium dioxide spin tips and graphite spin columns with specially formulated buffers.
Brand: Thermo Scientific™ 88301
Code : PN
Additional Details : CAS Number : 599-00-8 Weight : 5.44310kg Transport : UN number : 2699 Chem class : 8 Pack group : I
The complete kit includes 24 titanium dioxide (TiO2) spin tips and graphite spin columns with buffers to facilitate preparation of enriched and desalted phosphopeptides for analysis by mass spectrometry (MS).
- Convenient — spin-column format of titanium dioxide and graphite columns enable parallel processing and clean up of multiple samples in less than 2 hours
- High capacity — each column enriches up to 300μg of phosphopeptides
- Complementary — TiO2 enriches a unique set of phosphopeptides that complements the Pierce Fe-NTA IMAC Phosphopeptide Enrichment Kit
- High selectivity — recover phosphopeptides with >90% selectivity
The Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit protocol includes stringent washing conditions that increase the selectivity for phosphopeptides to >95%. Because some of the salts in these stringent washes may still be present in the eluted samples, the Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit includes graphite spin columns to desalt and concentrate enriched phosphopeptides, resulting in more successful phosphopeptide analysis results. This kit is compatible with samples digested in solution or after in-gel digestion with trypsin or other MS grade proteases. Using the Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit and the Pierce Fe-NTA IMAC Phosphopeptide Enrichment Kits will enable complementary sets of phosphopeptides to be removed from complex samples.
|Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit|
|24 rxn kit|
|Multi-component kit with TiO2 Spin Tips|
|TiO2 Spin Tips, 24 tips, Trifluoroacetic Acid, 1mL, Pyrrolidine, 200µL, Pierce Graphite Spin Columns, 24 columns, Centrifuge Column Adaptors, 6 adaptors, 90% Lactic Acid, 2mL|
|Processing 24 digested protein samples (0.3-1mg each) containing up to 100µg of phosphopeptides each|