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Thermo Scientific™ Pierce™ Sulfo-SBED Biotin Label Transfer Reagent

Chemically tag purified proteins at primary amines to covalently crosslink and transfer biotinylation to protein interaction binding partners.

Brand:  Thermo Scientific™ 33033

Code : PN

Additional Details : Weight : 1.81440kg

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Product Code. 11899240

  • £206.00 / 10mg


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Description

Description

Ready-to-use kit eliminates the need to prepare the necessary buffers or purchase the critical reagents necessary to perform a label transfer experiment. Key reagents, Sulfo-SBED and DTT, are supplied in unique, no-weigh, single-use packaging.

Highlights:

  • Sulfo-NHS ester reacts with primary amines at pH 7-9
  • Aryl azide conjugates randomly when activated with UV light
  • Cleavable disulfide bond allows biotin label transfer
  • Discover protein interactions without radiolabeling

Includes:

No-Weigh Sulfo-SBED Label Transfer Reagent, 8 × 1mg; BupH Phosphate Buffered Saline, 1 Pk. (makes 500mL); Label Transfer Buffer (20X), 200mL (makes 4L); Streptavidin-HRP Conjugate, 0.1mg; No-Weigh Dithiothreitol (DTT), 8 × 7.7mg; Slide-A-Lyzer MINI Dialysis Units plus Float, 10K MWCO, 10-100μL, 10 units/Pk.

Specifications

Specifications

Amine-to-Nonselective
Pierce Sulfo-SBED Biotin Label Transfer Reagent
No
No
23mL of typical 1X (0.5mM) reaction solution, or approx. 5 uses at 2mg reagent per use
Yes
Yes
Sulfo-N-hydroxysuccinimidyl-2-(6-[biotinamido]-2-(p-azido benzamido)-hexanoamido) ethyl-1,3'-dithioproprionate; powder solid
879.98
10mg
14.3; 21.2; 24.7Å
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