Thermo Scientific™ Pierce™ SILAC Media and Sera

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 88423

UNSPSC: 41115404

Code: A0

Additional Details:
Additional Details: Weight: 1.81440kg



Product Code. 12162410

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Description and Specification

Specification

Formulation IMDM media minus L-Lysine and L-Arginine
Sufficient For Part of 1 SILAC experiment
Description IMDM for SILAC
For Use With (Application) SILAC experiments (stable isotope labeling with amino acids in cell culture)
Quantity 500mL

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Flexible–liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling

  • Compatible–DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat

  • Optimized, specialized media–the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

  • High quality–Stem Cell Screened Dialyzed FBS is tested to ensure that it does not promote stem cell differentiation