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Thermo Scientific™ Pierce™ SILAC Media and Sera

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Brand:  Thermo Scientific™ 88422

Code : A0

Additional Details : Weight : 1.81440kg

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Product Code. 12152410

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Description

Description

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Flexible–liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling

  • Compatible–DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat

  • Optimized, specialized media–the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

  • High quality–Stem Cell Screened Dialyzed FBS is tested to ensure that it does not promote stem cell differentiation

Specifications

Specifications

MEM for SILAC
SILAC experiments (stable isotope labeling with amino acids in cell culture)
Part of 1 SILAC experiment
MEM media minus L-Lysine and L-Arginine
500mL
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