Thermo Scientific™ Pierce™ SILAC Media and Sera

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 88420

X6 DMEM MEDIA FOR SILAC (DMEM MINUS L-LYSINE ANDL-Arginine)500mL Store in Fridge at 4C

UNSPSC: 41115404

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 12132410

Quantity Price
1 £ 194.0 / Pack of 6
EU Stock 14
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Description and Specification

Specification

Product Type DMEM Media For SILAC
Quantity 1 Each
Quantity 6 x 500mL
Storage Requirements 2 to 8°C
Sufficient For 1 to 3 SILAC experiments
Storage 2 to 8°C
Description DMEM Media for SILAC, 6 x 500mL, Cells Starting Material, Used With Mass Spectrometer, For Use With Stable Isotope Labeling With Amino Acids in Cell Culture (SILAC) To Analyze Protein Expression by Mass Spectrometry (MS)
For Use With (Equipment) Mass spectrometry
Formulation DMEM media minus L-Lysine and L-Arginine
For Use With (Application) Protein biology, protein quantitation using mass spectrometry, protein mass spectrometry analysis, SILAC experiments (stable isotope labeling with amino acids in cell culture)
For Use With SILAC experiments (stable isotope labeling with amino acids in cell culture)

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Flexible–liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling

  • Compatible–DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat

  • Optimized, specialized media–the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

  • High quality–Stem Cell Screened Dialyzed FBS is tested to ensure that it does not promote stem cell differentiation