Thermo Scientific™ Pierce™ RED Device for Rapid Equilibrium Dialysis
Perform plasma protein-binding assays, a critical step in drug development, with the easiest, fastest and most reliable rapid equilibrium dialysis (RED) system.
Brand: Thermo Scientific™ 89811
Code : PN
Additional Details : Weight : 0.10000kg
DescriptionThe RED Device is an apparatus for performing equilibrium dialysis experiments in a high throughput, automation-compatible format. The device consists of disposable inserts and a base plate formatted to a standard microplate footprint.
Each single-use, disposable insert is made of two side-by-side chambers separated by a vertical cylinder of dialysis membrane (8K MWCO) validated for minimal nonspecific binding. This format requires no extensive assembly steps or specialized equipment, and each chamber or well is easily accessible from the top of the insert after insertion in the base plate. Additionally, the high surface-to-volume ratio of the membrane compartment allows rapid dialysis, where equilibrium can be reached in 4 hours with high levels of reproducibility and accuracy. In many cases, experiments can be completed in less than 100 minutes.
RED Device Inserts are designed to be used with either the reusable PTFE or disposable high-density polypropylene base plates. Each RED Device Base Plate holds up to 48 RED Device Inserts and has a standard 96-well plate footprint with 9mm x 9mm well spacing to provide compatibility with automated liquid handling systems. In addition, the disposable RED Device Base Plates are available pre-loaded, providing operation convenience for scientists conducting protein-binding applications. No pre-conditioning of the membrane inserts is needed. When using radioactive materials, this single-use plate is easily disposed of to avoid contamination and cleaning. RED Device Inserts and Base Plates are also available separately.
Easy and ready to use – disposable tubes require no presoaking, assembly or specialized equipment
Designed for speed – the high surface-to-volume ratio of the insert design enables equilibrium to be reached in as few as 100 minutes with vigorous agitation or in three to four hours with 200rpm agitation
Automation-compatible – designed on a standard 96-well plate template suitable for automated liquid handlers
Flexible and scalable – perform any number of assays (1 to 48 assays per plate) without wasting the entire plate
Robust – compartmentalized design eliminates potential for cross talk or leakage
Reproducible and accurate – validated for plasma binding assays, producing results consistent with those reported in literature
Quality-tested – each lot of inserts is functionally tested in a protein-binding assay for guaranteed performance
- Disposable: require no presoaking, assembly, or specialized equipment
- Short incubation time: large dialysis surface area accelerates equilibrium
- 8K MWCO membrane: ideal molecular-weight cutoff for protein-drug binding studies
- Membrane composition: regenerated cellulose with low glycerol content as a humectant
Base Plate Features:
- Microplate footprint: compatible with automated systems for 96-well plates
- Compartmentalized: eliminates potential for crosstalk or leakage
- PTFE construction: eliminates nonspecific binding and risk of contamination
- Accepts 1 to 48 inserts: run exactly the number of assays needed without waste
Plasma protein-binding assays; Drug partition between plasma and whole blood; Determination of protein binding of liver microsomes to improve the correlation between in vitro and in vivo intrinsic clearance; Drug binding competition between tissues vs. plasma proteins; Pharmacokinetics studies; Formulation of drug dosage for in vivo studies; Drug-drug interaction studies; Selection criteria during drug lead optimization; Solubility studies; Dissociation constant determination (Kd); Tissue binding studies using tissue homogenate
|Pierce RED Device Reusable Base Plate|
|Unlimited (reusable) 48-well experiments with RED Inserts (sold separately)|
|Reusable Base Plate; 1 plate|