Thermo Scientific Pierce™ Recombinant Protein G and Conjugates

Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.

Overview
Brand: Thermo Scientific

Manufacturer Part Number: 77675

50MG PIERCE RECOMBINANT PROTEIN G

UNSPSC: 41105300

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 15285683

Quantity Price
1 £ 644.0 / 50mg
Estimated Shipment date
from Supplier 02-01-2017
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Description and Specification

Specification

Formulation Salt-free powder
Quantity 50mg

Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2.

Highlights:

  • Contains two Fc-binding domains per protein
  • Better than Protein A for mouse (incl. IgG1), human (incl. IgG3) and rat, goat and cow antibodies
  • Poorer than Protein A for guinea pig, pig, dog and cat
  • Does not bind human IgM, IgD or IgA