Thermo Scientific™ Pierce™ Recombinant Protein G and Conjugates

Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 21193

5 MG PURIFIED RECOMB PROTEIN G

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 1.81440kg



Product Code. 10712725

Quantity Price
1 £ 325.0 / 5mg
EU Stock 8
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Description and Specification

Specification

Description Pierce Recombinant Protein G
Formulation Salt-free powder
Quantity 5mg

Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2.

Highlights:

  • Contains two Fc-binding domains per protein
  • Better than Protein A for mouse (incl. IgG1), human (incl. IgG3) and rat, goat and cow antibodies
  • Poorer than Protein A for guinea pig, pig, dog and cat
  • Does not bind human IgM, IgD or IgA