Improvement in sequence coverage and overall protein characterization can result from improving peptide chromatographic or ionization properties. The masses of these larger peptides can be accurately measured at high charge states on popular high-resolution mass spectrometers, reducing the database search time and increasing the sequence coverage and confidence. Finally, larger peptides can be fragmented effectively by new fragmentation techniques like electron transfer dissociation (ETD) or electron capture dissociation (ECD) that leave post-translational modifications, like phosphorylation, intact.
Proteases offer specific cleavage at multiple sites, enabling exploration of primary protein structure
Better protein characterization results from overlapping peptides with complementary chromatographic, ionization and fragmentation properties
Protocols are designed for MS applications
Streamlined data processing and analysis improves interpretation and confidence