Thermo Scientific Pierce™ Premium Grade EDC

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this popular carbodiimide crosslinker.

Overview
Brand: Thermo Scientific

Manufacturer Part Number: PG82073

25GR PREMIUM GRADE EDC

UNSPSC: 12161502

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 15235763

Quantity Price
1 £ 311.0 / 25g
Estimated Shipment date
from Supplier 15-12-2016
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Description and Specification

Specification

Formulation 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; crystalline powder
Quantity 25g
Sufficient For 62.5L of typical 1X (2mM) reaction solution

Thermo Scientific Pierce Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade EDC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. EDC is 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride, a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation. In the presence of N-hydroxysulfosuccinimide (sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding sulfo-NHS.

Highlights:

  • EDC – carbodiimide crosslinker for conjugation between carboxylates and amines
  • High quality – identity and purity confirmed by several tests
  • Product integrity – enhanced level of testing and characterization compared to standard grade
  • Lot retention – ample supply of past lots retained to ensure future process testing
  • Change management – Change Control Notification (CCN) service
  • Consistent manufacture – batch-specific manufacturing documentation review

Applications:

  • Conjugate carboxyl and amino groups among peptides and proteins
  • Couple haptens to immunogenic carrier proteins (e.g., attach a peptide to KLH)
  • Immobilize peptide antigens to affinity-purify antibodies
  • Create NHS-activated, amine-reactive labeling compounds
  • Crosslink proteins to carboxyl coated beads or surfaces
  • Activate nanoparticles with amine-reactive sulfo-NHS esters