Thermo Scientific™ Pierce™ PNPP Substrates

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Brand: Thermo Scientific™

Manufacturer Part Number: 34045

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: CAS Number: 1308-06-1 Weight: 0.80000kg

Product Code. 11889260

Quantity Price
1 £ 126.0 / 25g
EU Stock 1
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Description and Specification


Formulation p-Nitrophenyl phosphate disodium salt; powder
Sufficient For Preparing 25L of 1X (10mg/10mL) substrate buffer
Description Pierce PNPP Powder
Quantity 25g

PNPP (p-nitrophenyl phosphate, disodium salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution and a complete kit containing substrate solution and diethanolamine buffer.


  • Absorbance maximum of 505nm for measurement by ELISA plate readers
  • Develops a yellow hue in solution upon reaction with alkaline phosphatase (AP)
  • Safer to use – solution contains a non-mercury, non-azide preservative


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