Thermo Scientific™ Pierce™ PNPP Substrates
Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.
Brand: Thermo Scientific™ 34045
Code : PN
Additional Details : CAS Number : 1308-06-1 Weight : 0.80000kg
DescriptionPNPP (p-nitrophenyl phosphate, disodium salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution and a complete kit containing substrate solution and diethanolamine buffer.
- Absorbance maximum of 505nm for measurement by ELISA plate readers
- Develops a yellow hue in solution upon reaction with alkaline phosphatase (AP)
- Safer to use – solution contains a non-mercury, non-azide preservative
|Pierce PNPP Powder|
|p-Nitrophenyl phosphate disodium salt; powder|
|Preparing 25L of 1X (10mg/10mL) substrate buffer|