missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ PNPP Substrates

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Brand:  Thermo Scientific™ 34047

Code : PN

Additional Details : CAS Number : 1308-06-1 Weight : 0.80000kg

 View more versions of this product

Product Code. 11859270

  • £133.43 / 105 tablets

EU Stock 8
Add to basket



PNPP (p-nitrophenyl phosphate, disodium salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution and a complete kit containing substrate solution and diethanolamine buffer.


  • Absorbance maximum of 505nm for measurement by ELISA plate readers
  • Develops a yellow hue in solution upon reaction with alkaline phosphatase (AP)
  • Safer to use – solution contains a non-mercury, non-azide preservative


Pierce PNPP Tablets
Tablets containing 5mg of p-nitrophenyl phosphate disodium salt with inert filler as the balance
105 tablets
Preparing 5mL of 1X substrate buffer with each tablet

Technical Documents