Thermo Scientific™ Pierce™ PNPP Substrates

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Brand: Thermo Scientific™

Manufacturer Part Number: 34047

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: CAS Number: 1308-06-1 Weight: 0.80000kg

Product Code. 11859270

Quantity Price
1 £ 129.0 / 105 tablets
EU Stock 3
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Description and Specification


Formulation Tablets containing 5mg of p-nitrophenyl phosphate disodium salt with inert filler as the balance
Sufficient For Preparing 5mL of 1X substrate buffer with each tablet
Description Pierce PNPP Tablets
Quantity 105 tablets
Item Description PNPP Tablets, 5mg

PNPP (p-nitrophenyl phosphate, disodium salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution and a complete kit containing substrate solution and diethanolamine buffer.


  • Absorbance maximum of 505nm for measurement by ELISA plate readers
  • Develops a yellow hue in solution upon reaction with alkaline phosphatase (AP)
  • Safer to use – solution contains a non-mercury, non-azide preservative


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