Thermo Scientific™ Pierce™ PNPP Substrates

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Brand: Thermo Scientific™

Manufacturer Part Number: 34047


UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: CAS Number: 1308-06-1 Weight: 0.80000kg

Product Code. 11859270

Quantity Price
1 £123.00 / 105 tablets
EU Stock 3
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Description and Specification


Description Pierce PNPP Tablets
Formulation Tablets containing 5mg of p-nitrophenyl phosphate disodium salt with inert filler as the balance
Quantity 105 tablets
Sufficient For Preparing 5mL of 1X substrate buffer with each tablet

PNPP (p-nitrophenyl phosphate, disodium salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution and a complete kit containing substrate solution and diethanolamine buffer.


  • Absorbance maximum of 505nm for measurement by ELISA plate readers
  • Develops a yellow hue in solution upon reaction with alkaline phosphatase (AP)
  • Safer to use – solution contains a non-mercury, non-azide preservative


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