Thermo Scientific™ Pierce™ PNPP Substrates

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 37620

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 1.81440kg Transport: UN number : 1760 Chem class : 1 Pack group : III



Product Code. 10571384

Quantity Price
1 £ 164.0 / 1 set
EU Stock 1
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Description and Specification

Specification

Formulation Two-component kit with PNPP tablets and 5X substrate buffer
Kit Contents Diethanolamine Buffer, 225mL
Sufficient For Preparing 525mL of substrate (5mL with each tablet)
Description Pierce PNPP Substrate Kit
Quantity

105-tablet kit

PNPP (p-nitrophenyl phosphate, disodium salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution and a complete kit containing substrate solution and diethanolamine buffer.

Highlights:

  • Absorbance maximum of 505nm for measurement by ELISA plate readers
  • Develops a yellow hue in solution upon reaction with alkaline phosphatase (AP)
  • Safer to use – solution contains a non-mercury, non-azide preservative

Documentation

Technical documents