Thermo Scientific™ Pierce™ PNPP Substrates

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Brand: Thermo Scientific™

Manufacturer Part Number: 37620


UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg Transport: UN number : 1760 Chem class : 1 Pack group : III

Product Code. 10571384

Quantity Price
1 £158.00 / 1 set
EU Stock 4
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Description and Specification


Description Pierce PNPP Substrate Kit
Formulation Two-component kit with PNPP tablets and 5X substrate buffer
Kit Contents Diethanolamine Buffer, 225mL

105-tablet kit

Sufficient For Preparing 525mL of substrate (5mL with each tablet)

PNPP (p-nitrophenyl phosphate, disodium salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution and a complete kit containing substrate solution and diethanolamine buffer.


  • Absorbance maximum of 505nm for measurement by ELISA plate readers
  • Develops a yellow hue in solution upon reaction with alkaline phosphatase (AP)
  • Safer to use – solution contains a non-mercury, non-azide preservative


Technical documents