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Thermo Scientific™ Pierce™ PNPP Substrates

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Brand:  Thermo Scientific™ 37620

Code : PN

Additional Details : Weight : 1.81440kg Transport : UN number : 1760 Chem class : 8 Pack group : III

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Product Code. 10571384

  • £169.63 / 1 set

EU Stock 16
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PNPP (p-nitrophenyl phosphate, disodium salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution and a complete kit containing substrate solution and diethanolamine buffer.


  • Absorbance maximum of 505nm for measurement by ELISA plate readers
  • Develops a yellow hue in solution upon reaction with alkaline phosphatase (AP)
  • Safer to use – solution contains a non-mercury, non-azide preservative


Pierce PNPP Substrate Kit
PNPP Tablets, 105 tablets (5mg/tablet), Diethanolamine Buffer, 225mL
Preparing 525mL of substrate (5mL with each tablet)
Two-component kit with PNPP tablets and 5X substrate buffer

105-tablet kit


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