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Thermo Scientific™ Pierce™ NBT/BCIP Substrate Solutions

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

£105.00 - £237.00

Specifications

Storage Requirements 4°C
For Use With (Application) Alkaline phosphate detection
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Products 4
Product Code Brand Description Price Quantity & Availability  
Product Code Brand Description Price Quantity & Availability  
10046213 View Documents Thermo Scientific™
34035
Pierce NBT Substrate £144.00
1g View alternative products
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EU Stock 10
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10589463 View Documents Thermo Scientific™
34040
Pierce BCIP Substrate £237.00
1g View alternative products
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EU Stock 4
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10434175 View Documents Thermo Scientific™
34042
Pierce 1-Step NBT/BCIP Substrate Solution £166.00
250mL View alternative products
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EU Stock 92
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11819280 View Documents Thermo Scientific™
34070
Pierce 1-Step NBT/BCIP Plus Suppressor Substrate Solution £105.00
100mL View alternative products
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EU Stock 10
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Description

Description

The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).

Highlights:

  • AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples

  • Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph

  • Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor

1-Step™ NBT/BCIP

  • Ideal for immunoblotting applications
  • Minimal assay-to-assay variability

1-Step NBT/BCIP Plus Suppressor

  • Ideal for staining tissues assayed with calf intestinal alkaline phosphatase without the interference caused by endogenous phosphatases
  • Contains 1mM levamisole to inhibit endogenous phosphatase activity
Specifications

Specifications

4°C
Alkaline phosphate detection