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Thermo Scientific™ 1-Step™ NBT/BCIP plus Suppressor Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Brand:  Thermo Scientific™ 34070

90.00 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : PN

Additional Details : Weight : 0.80000kg

Product Code. 11819280

  • £135.00 / 100mL
Estimated Shipment: 09-04-2024
to see stock.
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Description

Description

Thermo Scientific™ Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).

Highlights:

  • AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples

  • Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph

  • Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor

1-Step™ NBT/BCIP

  • Ideal for immunoblotting applications
  • Minimal assay-to-assay variability

1-Step NBT/BCIP Plus Suppressor

  • Ideal for staining tissues assayed with calf intestinal alkaline phosphatase without the interference caused by endogenous phosphatases
  • Contains 1mM levamisole to inhibit endogenous phosphatase activity
Specifications

Specifications

Pierce 1-Step NBT/BCIP Plus Suppressor Substrate Solution
Colorimetric
Nitrocellulose, PVDF
100 mL
Alkaline Phosphatase
Substrate and Suppressor
Liquid
1-Step™
Alkaline Phosphatase
Upon receipt store at 4°C. Product is shipped with an ice pack.
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For Research Use Only. Not for use in diagnostic procedures.