Thermo Scientific™ Pierce™ Mass Spec Sample Prep Kit for Cultured Cells

Perform preparation of clean peptide mixtures from cultured cells for mass spectrometry (MS) analysis with this easy-to-use, comprehensive kit.

Brand: Thermo Scientific™

Manufacturer Part Number: 84841

10ug Digestion indicator for Mass spectrometry

UNSPSC: 41115404

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 13474219

Quantity Price
1 £153.00 / 10µg
Estimated Shipment date
from Supplier 07-11-2016
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Description and Specification


Description Pierce Digestion Indicator for Mass Spectrometry
Quantity 10µg

The Pierce Mass Spec Sample Prep Kit for Cultured Cells contains all the necessary reagents and enzymes to prepare up to 20 samples (1 million cells each) for MS analysis. The simple and robust workflow uses a lysis protocol that generates approximately 100μg of protein per sample and includes a control digestion indicator to monitor the efficiency of the two-step enzymatic digestion protocol. The kit procedure, which includes a Digestion Indicator as an internal protein/peptide control, produces consistently and reproducibly clean peptide mixtures for protein identification.


  • Complete – includes all reagents, a digestion indicator control, proteases and an optimized protocol needed to process up to 20 samples

  • Simple – user friendly kit can provide reproducible results even for non-expert MS analysts

  • Flexible – can be adapted to handle sample sizes between 10 to 200μg

  • High yield – total protein yield from 1 million cells is greater than 100μg

  • Optimized – cysteine reduction and alkylation are 100% with less than 1% over alkylation of non-cysteine residues

  • Efficient - percentage of missed cleavages is less than 10%

  • Compatible – final preparation is ready for direct MS analysis and other downstream applications, including mass-tag labeling


Kit contains proprietary lysis and digestion buffers, digestion indicator, DTT, iodoacetamide, Lys-C, trypsin