The Pierce Mass Spec Sample Prep Kit for Cultured Cells contains all the necessary reagents and enzymes to prepare up to 20 samples (1 million cells each) for MS analysis. The simple and robust workflow uses a lysis protocol that generates approximately 100μg of protein per sample and includes a control digestion indicator to monitor the efficiency of the two-step enzymatic digestion protocol. The kit procedure, which includes a Digestion Indicator as an internal protein/peptide control, produces consistently and reproducibly clean peptide mixtures for protein identification.
Complete – includes all reagents, a digestion indicator control, proteases and an optimized protocol needed to process up to 20 samples
Simple – user friendly kit can provide reproducible results even for non-expert MS analysts
Flexible – can be adapted to handle sample sizes between 10 to 200μg
High yield – total protein yield from 1 million cells is greater than 100μg
Optimized – cysteine reduction and alkylation are 100% with less than 1% over alkylation of non-cysteine residues
Efficient - percentage of missed cleavages is less than 10%
Compatible – final preparation is ready for direct MS analysis and other downstream applications, including mass-tag labeling