Thermo Scientific™ Pierce™ Maleic Anhydride (Amine-binding) Plates

Bind amine-containing peptides and other molecules to microplate wells for use in binding assays with these maleic anhydride-activated plates.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 15112

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10712794

Quantity Price
1 £ 624.0 / Pack of 25
Estimated Shipment date
from Supplier 04-04-2017
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Description and Specification

Specification

Formulation Clear, 96-well plates, coated with 200uL of Maleic Anhydride and blocked with n/auL of n/a
Sufficient For Binding approx. 125pmol biotin-pentylamine per well
Description Pierce Maleic Anhydride Activated Plates, Clear, 96-Well
Binding Target Amine
Quantity 5 x 5 plates

Pierce Maleic Anhydride Activated Plates are useful for immobilizing peptides and other ligands that do not coat efficiently by passive adsorption. Reaction of the maleic anhydride groups with primary amines (–NH2) results in formation of amide bonds that are stable at neutral pH and above. Acidic conditions hydrolyze the bond; for example, the half-life of hydrolysis at pH 3.5 and 37°C is approximately 11 hours. Therefore, coating reactions are best performed at pH 8 to 9, and the reacted plates are best used at pH greater than 7 for ELISA and other methods.

Highlights:

  • Spontaneously react with primary amines
  • A direct, simple approach to covalently attach molecules to the well surfaces of assay plates
  • Maleic anhydride maintains its integrity and availability for coupling for months under dry, room temperature conditions

Documentation

Technical documents