missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit

Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.

Brand:  Thermo Scientific™ 88954

Code : X3

Additional Details : Weight : 0.45360kg

 View more versions of this product

Product Code. 13464269

  • £248.00 / 1000 reactions

EU Stock 29
Add to basket



The Pierce LDH Cytotoxicity Assay Kit measures extracellular LDH in culture media using an enzymatic reaction that results in a red formazan product which can be measured spectrophotometrically. Lactate dehydrogenase (LDH) is a cytosolic enzyme that is is an indicator of cellular toxicity. The assay is ideal for high-throughput screening and provides a safe alternative to radioactive cytotoxicity assays.


  • Convenient – add–mix–read assay format for adherent and suspension cells

  • Colorimetric – quantitatively measures LDH release by formation of colored product

  • Robust – uses stable LDH enzyme activity as a cytotoxic marker

  • Flexible – ideal for high–throughput screening

  • Non–radioactive – safe alternative to 51Cr–release cytotoxicity assays


Kit contains substrate mix, assay buffer, 10X lysis buffer, stop solution, and LDH positive control


Microplate reader capable of reading absorbance at 490nm and 680nm, flat-bottom clear 96–well plate compatible with spectrophotometer, multichannel pipette

Recommended for:

  • Measure in vitro cytotoxicity mediated by chemicals, immune cells, or siRNA or microRNA
  • Detect cytolysis in bioreactor or 3D tissue engineering applications


Pierce LDH Cytotoxicity Assay Kit
10X Lysis Buffer, 12mL, Substrate Mix, 5 x lyophilizate, Stop Solution, 60mL, LDH Positive Control, 30┬ÁL, Assay Buffer, 5 x 0.6mL
1000 reactions in 96-well plates or 4000 reactions in 384-well plates
Five-component kit
1000 rxn kit