Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit

Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 88954

1000RXN LDH Cytotoxicity assay kit

UNSPSC: 41116126

Code: X3

Additional Details:
Additional Details: Weight: 0.45360kg



Product Code. 13464269

Quantity Price
1 £ 223.0 / 1000 reactions
EU Stock 43
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Description and Specification

Specification

Formulation Five-component kit
Kit Contents 10X Lysis Buffer, 12mL
Sufficient For 1000 reactions in 96-well plates or 4000 reactions in 384-well plates
Description Pierce LDH Cytotoxicity Assay Kit
Quantity 1000 rxn kit

The Pierce LDH Cytotoxicity Assay Kit measures extracellular LDH in culture media using an enzymatic reaction that results in a red formazan product which can be measured spectrophotometrically. Lactate dehydrogenase (LDH) is a cytosolic enzyme that is is an indicator of cellular toxicity. The assay is ideal for high-throughput screening and provides a safe alternative to radioactive cytotoxicity assays.

Highlights:

  • Convenient – add–mix–read assay format for adherent and suspension cells

  • Colorimetric – quantitatively measures LDH release by formation of colored product

  • Robust – uses stable LDH enzyme activity as a cytotoxic marker

  • Flexible – ideal for high–throughput screening

  • Non–radioactive – safe alternative to 51Cr–release cytotoxicity assays

Includes:

Kit contains substrate mix, assay buffer, 10X lysis buffer, stop solution, and LDH positive control

Requires:

Microplate reader capable of reading absorbance at 490nm and 680nm, flat-bottom clear 96–well plate compatible with spectrophotometer, multichannel pipette

Recommended for:

  • Measure in vitro cytotoxicity mediated by chemicals, immune cells, or siRNA or microRNA
  • Detect cytolysis in bioreactor or 3D tissue engineering applications