Thermo Scientific™ Pierce™ IP Lysis Buffer

Maximize cell lysate yield, purity and compatibility with this immunoprecipitation-optimized protein extraction reagent.

Brand: Thermo Scientific™

Manufacturer Part Number: 87788

250 ML PIERCE IP LYSIS BUFFER FOR 25,000CM2 MONOLAyer or 25g cell paste 250mL Store in fridge at 4C

UNSPSC: 41116012

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 11825135

Quantity Price
1 £154.00 / 250mL
EU Stock 7
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Description and Specification


Description Pierce IP Lysis Buffer
Formulation 25mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% NP-40 and 5% glycerol
Quantity 250mL
Sufficient For 25,000cm2 of cell monolayer or 25g of cell paste
Format Liquid
Source Material Cultured mammalian cells

The IP Lysis Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This moderate-strength lysis buffer effectively solubilizes cellular proteins but does not liberate genomic DNA or disrupt protein complexes like ordinary RIPA buffer. Pierce IP Lysis Buffer is specially formulated for pulldown and immunoprecipitation assays.


  • Optimized for compatibility with immunoprecipitation and pull-down assays
  • Compatible with protein assays, reporter assays and immunoassay procedures
  • Ready-made formula is effective for extracting cytoplasmic, membrane and nuclear proteins
  • Gentle formulation helps maintain protein complexes for co-immunoprecipitation (co-IP)
  • Does not liberate genomic DNA which can cause high sample viscosity