Thermo Scientific™ Pierce™ IP Lysis Buffer

Maximize cell lysate yield, purity and compatibility with this immunoprecipitation-optimized protein extraction reagent.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 87787

UNSPSC: 41116012

Code: PN

Additional Details:
Additional Details: Weight: 2.72160kg



Product Code. 11820084

Quantity Price
1 £ 80.25 / 100mL
EU Stock 22
Add to basket


Description and Specification

Specification

Formulation 25mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% NP-40 and 5% glycerol
Source Material Cultured mammalian cells
Sufficient For 10,000cm2 of cell monolayer or 10g of cell paste
Format Liquid
Description Pierce IP Lysis Buffer
Quantity 100mL

The IP Lysis Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This moderate-strength lysis buffer effectively solubilizes cellular proteins but does not liberate genomic DNA or disrupt protein complexes like ordinary RIPA buffer. Pierce IP Lysis Buffer is specially formulated for pulldown and immunoprecipitation assays.

Highlights:

  • Optimized for compatibility with immunoprecipitation and pull-down assays
  • Compatible with protein assays, reporter assays and immunoassay procedures
  • Ready-made formula is effective for extracting cytoplasmic, membrane and nuclear proteins
  • Gentle formulation helps maintain protein complexes for co-immunoprecipitation (co-IP)
  • Does not liberate genomic DNA which can cause high sample viscosity