Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Brand: Thermo Scientific™ 88946
Code : PN
Additional Details : Weight : 0.01000kg
DescriptionPierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln▾Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design.
Effective – HRV 3C is designed to remove purification tags from your protein of interest
High activity at 4°
C – activity is ≥2000U/mg control protein; one unit will cleave 0.1mg control protein at 4°C after 16h
Flexible – dual-tagged enzyme is compatible with various columns and buffers
Kit contains protease, cleavage buffer and control protein.
Removal of purification tags from fusion proteins
|Pierce HRV 3C Protease|
|HRV3C Protease (2 units/µL), 500uL, GST-Syk Fusion Protein (1µg/µL), 25 µL, Reaction Buffer (10X), 10mL|
|Cleavage of 100mg of target protein at 1:200 enzyme-to-substrate ratio|