Cleavage of 100mg of target protein at 1:200 enzyme-to-substrate ratio
Pierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln▾Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design.
Effective – HRV 3C is designed to remove purification tags from your protein of interest
High activity at 4°
C – activity is ≥2000U/mg control protein; one unit will cleave 0.1mg control protein at 4°C after 16h
Flexible – dual-tagged enzyme is compatible with various columns and buffers
Kit contains protease, cleavage buffer and control protein.