Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/µL)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Brand: Thermo Scientific™ 88947
Code : PN
Additional Details : Weight : 0.01000kg
DescriptionPierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln▾Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design.
Effective – HRV 3C is designed to remove purification tags from your protein of interest
High activity at 4°
C – activity is ≥2000U/mg control protein; one unit will cleave 0.1mg control protein at 4°C after 16h
Flexible – dual-tagged enzyme is compatible with various columns and buffers
Kit contains protease, cleavage buffer and control protein.
Removal of purification tags from fusion proteins
|Store at -20°C.|
|10K Unit Kit|