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Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/µL)

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Brand:  Thermo Scientific™ 88947

Code : PN

Additional Details : Weight : 0.01000kg

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Product Code. 13454289

  • £1986.00 / 10ku


Estimated Shipment
12-10-2020
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Description

Description

Pierce HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln▾Gly-Pro and cleaves after the glutamine residue. The recombinant enzyme is engineered as a fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so that the protease can be easily removed after cleavage using a variety of solid supports with immobilized glutathione (GSH) or metal chelates, respectively. HRV3C is also active in a variety of commonly used buffers, providing further flexibility in experimental design.

Highlights:

  • Effective – HRV 3C is designed to remove purification tags from your protein of interest

  • High activity at 4°

    C – activity is ≥2000U/mg control protein; one unit will cleave 0.1mg control protein at 4°C after 16h

  • Flexible – dual-tagged enzyme is compatible with various columns and buffers

Includes:

Kit contains protease, cleavage buffer and control protein.

Recommended for:

Removal of purification tags from fusion proteins

Specifications

Specifications

Store at -20°C.
Kit
Pierce™
10K Unit Kit
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