Thermo Scientific™ Pierce™ Horseradish Peroxidase (HRP)

Purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 31491

IMMUNOPURE HORSERADISH PEROX 100MG

UNSPSC: 41115823

Code: PN

Additional Details:
Additional Details: CAS Number: 1436-34-6 Weight: 1.81440kg



Product Code. 11869150

Quantity Price
1 £ 235.0 / 100mg
EU Stock 4
Add to basket


Description and Specification

Specification

Quantity 100mg
Item Description 100mg
Formulation Salt-free lyophilized powder of horseradish peroxidase
Sufficient For Many uses depending on the application

Highlights:

  • Superior to alkaline phosphatase and β-galactosidase conjugates due to the higher specific enzyme activity
  • Small size (40kDa) allows excellent cellular penetration
  • Variety of substrates available
  • Ideal in blotting and cytochemistry applications
  • Used as the reporter enzyme for Thermo Scientific SuperSignal Chemiluminescent Western Blotting and ELISA Substrates

Note:

Porstmann, B., Porstmann, T., Nugel, E. and Evers, U. (1985). Which of the commonly used marker enzymes gives the best results in colorimetric and fluorimetric enzyme immunoassays: horseradish peroxidase, alkaline phosphatase, β-galactosidase, J. Immunol. Meth. 79, 27-37.

Wordinger, R.J., Miller, G.W. and Nicodemus, D.S. (1987). Manual of Immunoperoxidase Techniques, 2nd Edition. Chicago: American Society of Clinical Pathologists Press, pp. 23-24.

Yolken, R.H. (1982). Enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects. Rev. Infect. Dis. 4(1), 35-68.

Cordell, J.L., et al. (1984). Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J. Histochem. Cytochem. 32, 219-229.

Passey, R.B., et al. (1977). Evaluation and comparison of 10 glucose methods and the reference method recommended in the proposed product class standard. Clin. Chem. 23(1), 131.

Hosoda, H., Takasaki, W., Tsukamoto, R. and Nambara, T. (1987). Sensitivity of steroid immunoassays. Comparison of alkaline phosphatase, β-galactosidase and horseradish peroxidase as labels in a colorimetric assay system. Chem. Pharm. Bull. 35, 3336-3342.

Samoszuk, M.K., et al. (1989). Antibody, Immunoconjugates and Radiopharmaceuticals 2, 37-46.