Thermo Scientific™ Pierce™ Glutathione Superflow Agarose
Perform high-purity, large-scale purification of GST-tagged proteins with glutathione that has been immobilized to a crosslinked 6% beaded agarose.
Brand: Thermo Scientific™ 25239
Code : PN
Additional Details : Weight : 0.01000kg
DescriptionPierce Glutathione Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates required for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemical and pH values, and it is compatible with common clean-in-place procedures. The specific binding of glutathione to the GST-tag results in high purity and high yield in a single step purification process.
High dynamic binding capacity – binds 10mg of pure GST per mL of resin at a linear flow rate of 150cm/hour; can bind greater than 20mg/mL at 30cm/hour flow rate
High purity – provides greater than 90% purity when used to purify overexpressed GST or GST-fusion proteins from lysates
Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour
Compatible – maintains function after exposure to a wide variety of chemicals and pH values
Cost effective – resin is stable through multiple cycles of cleaning and reuse
Medium- to large-scale FPLC purification of GST-tagged proteins
|Pierce Glutathione Superflow Agarose|
|Glutathione onhighly crosslinked 6% beaded agarose (superflow 6), slurried in water with sodium azide|
|Binding up to 30mg GST/mL resin (static capacity) or up to 10mg GST/mL resin (dynamic capacity)|