missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ Glutathione Magnetic Beads

Affinity purify GST-tagged fusion proteins from cell lysates using glutathione (GSH) magnetic particles for manual or robotic magnetic separation devices.

£113.04 - £380.00

Specifications

Description Pierce Glutathione Magnetic Beads
Formulation Glutathione on paramagnetic iron oxide particles (1 to 10um dia.) slurried in 20% ethyl alcohol
Support Type Magnetic Bead
Sufficient For Binding 5 to 10mg GST per mL of beads
View More Specs

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
11824131
View Documents Promotion Details
Thermo Scientific™
88821
4 mL This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A View alternative products
Save Up to 
»
11834131
View Documents Promotion Details
Thermo Scientific™
88822
20 mL This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A View alternative products
Save Up to 
»
Description

Description

Thermo Scientific™ Pierce Glutathione Magnetic Beads are superior GST-binding particles for automated magnetic separations.
Additional Information and Online Ordering »

Glutathione magnetic beads provide a simple, rapid and reliable means of purifying glutathione S-transferase (GST) fusion proteins from crude cell lysates prepared from bacteria, yeast or mammalian cells. The protocol for GST-tagged protein purification is simple: the magnetic particles are added to a lysate containing the GST-fusion protein, and after incubation, non-bound proteins are removed by washing and the GST-fusion proteins are eluted using reduced glutathione. The high-performance iron oxide superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher™ 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.

Highlights:

  • High-performance beads – non-aggregating, silanized, iron oxide, paramagnetic microparticles provide exceptional uniformity for automated HTS applications

  • Stable affinity ligand – glutathione is covalently immobilized to particles, ensuring leach-resistance and clean purification products

  • High capacity – binding capacity is sufficient for both routine and demanding magnetic separation procedures

  • GST-tag affinity – standard glutathione-GST affinity purification chemistry in a magnetic format; bind in physiologic buffers and elute with 50mM reduced glutathione (prepared with Part No. 78259)

Properties of Glutathione Magnetic Beads:

  • Composition: iron oxide particles with reduced glutathione (GSH) covalently attached to the surface and encapsulated by crosslinked agarose
  • Magnetization: superparamagnetic (no magnetic memory)
  • Bead size: 1 to 10μm (mean diameter)
  • Concentration: 25% slurry (based on volume) in 20% ethanol
  • Binding capacity: 5 to 10mg of GST fusion protein per milliliter of settled beads (i.e., 4mL of 25% slurry)

Requires:

Thermo Scientific KingFisher 96 or KingFisher Flex Instrument or other magnetic separator device

Specifications

Specifications

Pierce Glutathione Magnetic Beads
Glutathione on paramagnetic iron oxide particles (1 to 10um dia.) slurried in 20% ethyl alcohol
Magnetic Bead
Binding 5 to 10mg GST per mL of beads
Videos
SDS
Documents

Documents

Product Certifications