missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ Glutathione Magnetic Beads

Affinity purify GST-tagged fusion proteins from cell lysates using glutathione (GSH) magnetic particles for manual or robotic magnetic separation devices.

Brand:  Thermo Scientific™ 88822

Code : PN

Additional Details : CAS Number : 306936-03-8 Weight : 0.80000kg

 View more versions of this product

Product Code. 11834131

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
View alternative products »



Glutathione magnetic beads provide a simple, rapid and reliable means of purifying glutathione S-transferase (GST) fusion proteins from crude cell lysates prepared from bacteria, yeast or mammalian cells. The protocol for GST-tagged protein purification is simple: the magnetic particles are added to a lysate containing the GST-fusion protein, and after incubation, non-bound proteins are removed by washing and the GST-fusion proteins are eluted using reduced glutathione. The high-performance iron oxide superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher™ 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.


  • High-performance beads – non-aggregating, silanized, iron oxide, paramagnetic microparticles provide exceptional uniformity for automated HTS applications

  • Stable affinity ligand – glutathione is covalently immobilized to particles, ensuring leach-resistance and clean purification products

  • High capacity – binding capacity is sufficient for both routine and demanding magnetic separation procedures

  • GST-tag affinity – standard glutathione-GST affinity purification chemistry in a magnetic format; bind in physiologic buffers and elute with 50mM reduced glutathione (prepared with Part No. 78259)

Properties of Glutathione Magnetic Beads:

  • Composition: iron oxide particles with reduced glutathione (GSH) covalently attached to the surface and encapsulated by crosslinked agarose
  • Magnetization: superparamagnetic (no magnetic memory)
  • Bead size: 1 to 10μm (mean diameter)
  • Concentration: 25% slurry (based on volume) in 20% ethanol
  • Binding capacity: 5 to 10mg of GST fusion protein per milliliter of settled beads (i.e., 4mL of 25% slurry)


Thermo Scientific KingFisher 96 or KingFisher Flex Instrument or other magnetic separator device



Pierce Glutathione Magnetic Beads
Magnetic Bead
Glutathione on paramagnetic iron oxide particles (1 to 10um dia.) slurried in 20% ethyl alcohol
Binding 5 to 10mg GST per mL of beads