Thermo Scientific™ Pierce™ FITC and Fluorescein Dyes and Labeling Kits

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).

Brand: Thermo Scientific™

Manufacturer Part Number: 46410


UNSPSC: 41116012

Code: PN

Additional Details:
Additional Details: CAS Number: 306935-99-9 Weight: 0.00010kg

Product Code. 10698664

Quantity Price
1 £62.00 / 100mg
EU Stock 20
Add to basket

  • Description and Specification
  • Alternative Products
  • Documentation
Description and Specification


Description Pierce NHS-Fluorescein
Formulation N-hydroxysuccinimide ester activated fluorescein fluorescent dye
Sufficient For Approx. 50 uses to label a total of 1408mg of IgG using typical conditions
Quantity 100mg

Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.


  • Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery

Alternative Products

Technical documents