Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 16176

FIREFLY LUCIFERASE GLOW ASSAY KIT

UNSPSC: 41106500

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg



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Product Code. 12112250

Quantity Price
1 £ 89.75 / Each
EU Stock 4
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Description and Specification

Specification

Description Pierce Firefly Luciferase Glow Assay Kit
Formulation Three-component kit
Kit Contents D-Luciferin, 3mg
Quantity 100 rxn kit
Sufficient For 100 luciferase assays in microplate wells

This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of firefly luciferase in cell lysates. In the presence of firefly luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Firefly Luc Plasmids; however, this assay kit may be used to detect the activity of other firefly or ATP-dependent luciferases that use D-luciferin as a substrate.

Highlights:

  • Sensitive – highly sensitive detection of firefly luciferase activity; for best results (maximum signal) use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer

  • Stable – increased signal stability compared to flash assays

  • Simple – does not require a luminometer with injectors

  • Compatible – assay reagents compatible with other firefly beetle luciferases

  • Automation-friendly – amenable to high throughput assays

  • Convenient – contains a universal cell lysis buffer and optimized glow assay reagent

  • Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; For best results (maximum signal), use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies