Thermo Scientific™ Pierce™ Factor Xa

Protease (serine endopeptidase for prothrombin); cleaves arginine in the sequence Ile-Glu-Gly-Arg; useful for His-tagged fusion protein engineering.

Brand: Thermo Scientific™

Manufacturer Part Number: 32521


UNSPSC: 12161502

Code: PN

Additional Details:
Additional Details: Weight: 0.80000kg

Product Code. 11849240

Quantity Price
1 £122.00 / 50µg
EU Stock 1
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Description and Specification


Description Pierce Factor Xa
Formulation At least 1mg/mL enzyme in MES-buffered saline pH 6 with 1mM benzamidine
MS-Compatible No
Quantity 50µg
Sufficient For At least 250 units of activity/mg
Storage Requirements -70°C

Cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg and will occasionally cleave at other basic residues. However, it will not cleave at a site followed by proline or arginine. Often used to remove fusion tags, such as the common histidine tag, from expressed proteins.


  • Extremely specific proteolytic activity - useful in the genetic engineering of proteins
  • Cleaves after the last amino acid in the sequence Ile-Glu-Gly-Arg
  • Activity is >130U/mg using a synthetic Ile-Glu-Gly-Arg peptide with p-nitroanilide derivatized arginine as the substrate; each unit of enzyme cleaves 1mol/minute of substrate at 37°C in 5mM MES, 0.5M NaCl, 1mM benzamidine at pH 6 with 1% BSA

Additional Features:

  • Source: Bovine Plasma
  • Activity: 125units/mg
  • Supplied: ≥5mM MES, pH 6, 0.5M NaCl
  • Protein Concentration: 1-2mg/mL

Ordering Information:

Supplied as frozen liquid.


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