Thermo Scientific™ Pierce™ Factor Xa

Protease (serine endopeptidase for prothrombin); cleaves arginine in the sequence Ile-Glu-Gly-Arg; useful for His-tagged fusion protein engineering.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 32520

FACTOR XA(BOVINE)250µG

UNSPSC: 12161502

Code: PN

Additional Details:
Additional Details: Weight: 0.80000kg



Product Code. 11839240

Quantity Price
1 £ 361.0 / 250µg
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

Description Pierce Factor Xa
Formulation At least 1mg/mL enzyme in MES-buffered saline pH 6 with 1mM benzamidine
MS-Compatible No
Quantity 250µg
Sufficient For At least 250 units of activity/mg
Storage Requirements -70°C

Cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg and will occasionally cleave at other basic residues. However, it will not cleave at a site followed by proline or arginine. Often used to remove fusion tags, such as the common histidine tag, from expressed proteins.

Highlights:

  • Extremely specific proteolytic activity - useful in the genetic engineering of proteins
  • Cleaves after the last amino acid in the sequence Ile-Glu-Gly-Arg
  • Activity is >130U/mg using a synthetic Ile-Glu-Gly-Arg peptide with p-nitroanilide derivatized arginine as the substrate; each unit of enzyme cleaves 1mol/minute of substrate at 37°C in 5mM MES, 0.5M NaCl, 1mM benzamidine at pH 6 with 1% BSA

Additional Features:

  • Source: Bovine Plasma
  • Activity: 125units/mg
  • Supplied: ≥5mM MES, pH 6, 0.5M NaCl
  • Protein Concentration: 1-2mg/mL

Ordering Information:

Supplied as frozen liquid.

Documentation

Technical documents