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Invitrogen™ ERK1/ERK2 Multispecies In-Cell ELISA Kit, Near Infrared

In-cell ELISA Kit

Brand:  Invitrogen™ 62211

Code : PN

Additional Details : Weight : 0.01000kg

Product Code. 11854035

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Description

Description

The ERK1/2 Multispecies In-Cell ELISA Near Infrared Detection Kits provide a simple and convenient method for quantifying intracellular proteins in whole cells. Principle of the method This kit enables simultaneous detection of post-translational-modified protein (PTM) and the corresponding unmodified protein, or two different targets within the same well. Simultaneously detecting targets within an ELISA well eliminates variability caused by differences in cell plating. The expression levels of the protein(s) are monitored using primary antibodies specific to the targets and corresponding species-specific near-infrared Thermo Scientific DyLight-Conjugated Secondary Antibodies. Relative protein activation or inactivation is determined as a ratio of modified protein levels to the corresponding unmodified protein measured using a modification-specific antibody and an antibody to unmodified protein, respectively. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

ERK1 and ERK2 (extracellular signal-regulated kinase 1 and 2) are widely involved in the regulation of meiosis, mitosis, and post-mitotic functions in cells. ERK1 and ERK2 are members of the MAP kinase family, which act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. Different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. Growth factors binding to the tyrosine kinase receptors, Ras/Raf activation, and serine/threonine protein kinase stimulation activate the ERK1/ERK2 pathways as well. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187, and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at the Threonine 202 and Tyrosine 204 residues of ERK1, and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. The ERK family has three additional members: ERK3, ERK5 and ERK6.
Specifications

Specifications

P27361, P28482
Unconjugated
ELISA Kit
96 microplate wells of overnight-cultured cells
Fluorescent Microplate Reader
ERK,ERK-2,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk,p42-MAPK,ERK-1,ERK1,ERT2,HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,p44-ERK1,p44-MAPK
DyLight™ 680, DyLight™ 800
RUO
2°C to 8°C
1 hr. 20 min.
Pierce ERK1/2 Near Infrared In-Cell ELISA Kit
Multi-component kit with fluorescent conjugates
Supernatant
Human
5594,5595
Blocking Buffer, 20X Tris Buffered Saline, Surfact-Amps 20, Surfact-Amps X-100, DyLight™ 680 Goat Anti-Mouse IgG (H+L), DyLight 800 Goat Anti-Rabbit IgG (H+L), Thin Plate Seal Assembly, Components included only in the target-specific kits:Antibody #1 and Antibody #2, 20X Tris Buffered Saline, 50mL
96 Tests
Supernatant, 10,000 cells
Human
24 hr.
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