Thermo Scientific™ Pierce™ EDC Crosslinker

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 77149

EDC(SUPERCARRIER IMMUNEMODULA)10MG

UNSPSC: 12161502

Code: PN

Additional Details:
Additional Details: CAS Number: 93-48-1 Weight: 1.81440kg



Product Code. 11844071

Quantity Price
1 £ 45.33 / 10mg
EU Stock 94
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Description and Specification

Specification

Spacer Arm Length
Formulation 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; crystalline powder
Cleavable No
Molecular Weight 191.7
Sufficient For 26mL of typical 1X (2mM) reaction solution, or approx. 5 uses at 2mg reagent per use
Description Pierce EDC
Membrane Permeable No
PEGylated No
Chemical Reactivity Carboxyl-to-Amine
Water Soluble Yes
Quantity 10mg

EDC is the abbreviation for 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, a crosslinker that has been used in diverse applications such as forming amide bonds in peptide synthesis, attaching haptens to carrier proteins to form immunogens, labeling nucleic acids through 5Æ phosphate groups and creating amine-reactive NHS-esters of biomolecules. EDC reacts with a carboxyl to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide (Sulfo-NHS, Part No. 24510), EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters.

Highlights:

  • Zero-length crosslinker
  • Numerous chemical conjugates can be synthesized via available –COOH and –NH2 groups
  • Amide bond formed provides a neutral linkage, which is ideal for preparing peptides and antigens
  • Adding Sulfo-NHS (Part No. 24510) enhances the coupling reaction at physiological pH conditions
  • Reactive groups: carbodiimide
  • Reactive toward: carboxyl and amino groups

Documentation

Technical documents