Thermo Scientific™ Pierce™ Cysteine-Reactive Tandem Mass Tag Reagents

Perform measurement of protein and peptide cysteine modifications (S-nitrosylation, oxidation and disulfide bridges) by multiplex quantitative mass spectrometry.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 90101

6RXN iodoTMTsixplex label reagent set

UNSPSC: 41115404

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 13414289

Quantity Price
1 £ 516.0 / 6 reactions
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

Description iodoTMTsixplex Label Reagent Set, 1 x 0.2mg
Formulation Set of -126 to -131 iodoTMT6 label reagents
Kit Contents iodoTMT6-126 Label Reagent, 0.2mg
Quantity 6 rxn kit
Sufficient For 1 sixplex (1 x 6-way) experiment

Tandem Mass Tag™ (TMT™) Reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry. The iodoTMT™ Reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (-SH) groups. Similar to iodoacetamide, iodoTMT Reagents react specifically with reduced cysteines (Cys) in peptides and proteins. IodoTMT Reagents can be differentiated by mass spectrometry (MS), enabling quantitation of the relative abundance of cysteine modifications, such as S–nitrosylation, oxidation and disulfide bonds, in cultured cells grown or treated with different conditions.

Highlights:

  • Specific – only reacts with sulfhydryl groups (reduced, unmodified cysteine residues)

  • Irreversible – labeled proteins and peptides are not susceptible to reducing agents

  • Flexible – options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation

  • Versatile – can be used to study cysteine modifications (e.g. di-sulfide bridges, S-nitrosylation)

Note:

IodoTMT Products replace our previously offered CysTMT Reagents, which utilized a dithiopyridine reactive group to reversibly label cysteine sulfhydryls.