Thermo Scientific™ Pierce™ Cypridina Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 16170

CYPRIDINA LUCIFERASE GLOW 100 RXN

UNSPSC: 41106500

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 12201622

Quantity Price
1 £ 86.0 / Each
Estimated Shipment date
from Supplier 14-12-2016
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Description and Specification

Specification

Description Pierce Cypridina Luciferase Glow Assay Kit
Formulation Three-component kit
Kit Contents 100X Vargulin, 50uL
Quantity 100 rxn kit
Sufficient For 100 luciferase assays in microplate wells

This Pierce Luciferase Glow Assay Kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Cypridina luciferases or derivatives that use Vargulin as a substrate.

Highlights:

  • Sensitive – highly sensitive detection of Cypridina luciferase activity

  • Secreted luciferase – allows real-time assays and kinetic studies without destroying cells

  • Stable – reagents provide increased signal stability compared to flash assays

  • Simple – does not require a luminometer with injectors

  • Compatible – assay reagents compatible with other Cypridina or Vargula luciferases

  • Automation-friendly – amenable to high throughput assays

  • Convenient – contains a universal cell lysis buffer and optimized glow assay reagent

  • Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies