The IgY Purification Kit is simple and easy to use. After isolating a yolk using the supplied egg separator, lipids are effectively removed by incubation and centrifugation in Pierce Delipidation Reagent. This first reagent is also effective as a storage solution, enabling multiple yolks to be collected and stored for simultaneous extraction at a later date. Upon addition of the specialized Pierce IgY Precipitation Reagent and subsequent centrifugation, a nearly pure pellet of intact IgY results; it is easily dissolved in phosphate buffered saline (PBS) or other storage buffer. The purified IgY is ready for use in immunodetection methods or antigen-specific purification without interference from lipids.
Cost-effective – purifies twice as much IgY per egg than other popular kits, providing a lower cost-per-milligram of purified IgY
High purity – method yields chicken antibody that is 85 to 95% pure (based on SDS-PAGE analysis)
Easy to use – simple precipitation method purifies chicken IgY without affinity columns
Flexible protocol – eggs can be harvested and quickly stored in buffer for purification at a later date
Convenient – use eggs directly out of refrigerator; no need to equilibrate them to room temperature
Egg separator, delipidation reagent and IgY precipitation reagent