Thermo Scientific™ Pierce™ Chicken IgY Purification Kits and Reagents

Purify chicken antibodies from hen egg yolks with a unique delipidation reagent and precipitation reagent to selectively isolate the IgY fraction.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 44922

CHICKEN IGY PURIFICATION KIT

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10559673

Quantity Price
1 £ 734.0 / 1 set
Estimated Shipment date
from Supplier 31-01-2017
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Description and Specification

Specification

Formulation Multi-component kit
Kit Contents Delipidation Reagent, 5 x 500mL
Sufficient For Purifying chicken IgY from 25 egg yolks
Format Liquid
Description Pierce Chicken IgY Purification Kit
Target IgY
Quantity 5L kit

The IgY Purification Kit is simple and easy to use. After isolating a yolk using the supplied egg separator, lipids are effectively removed by incubation and centrifugation in Pierce Delipidation Reagent. This first reagent is also effective as a storage solution, enabling multiple yolks to be collected and stored for simultaneous extraction at a later date. Upon addition of the specialized Pierce IgY Precipitation Reagent and subsequent centrifugation, a nearly pure pellet of intact IgY results; it is easily dissolved in phosphate buffered saline (PBS) or other storage buffer. The purified IgY is ready for use in immunodetection methods or antigen-specific purification without interference from lipids.

Highlights:

  • Cost-effective – purifies twice as much IgY per egg than other popular kits, providing a lower cost-per-milligram of purified IgY

  • High purity – method yields chicken antibody that is 85 to 95% pure (based on SDS-PAGE analysis)

  • Easy to use – simple precipitation method purifies chicken IgY without affinity columns

  • Flexible protocol – eggs can be harvested and quickly stored in buffer for purification at a later date

  • Convenient – use eggs directly out of refrigerator; no need to equilibrate them to room temperature

Includes:

Egg separator, delipidation reagent and IgY precipitation reagent