Thermo Scientific™ Pierce™ BCA™ Protein Assay

Green-to-blue (A562nm), two-component, precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 23223

1 LT PROTEIN ASSAY REAGENT A, BCA 1000ML

UNSPSC: 41116012

Code: PN

Additional Details:
Additional Details: CAS Number: 84387-89-3 Weight: 0.80000kg



Product Code. 11881345

Quantity Price
1 £ 134.0 / 1000mL
EU Stock 77
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  • Description and Specification
  • Alternative Products
  • Documentation
Description and Specification

Specification

Description Pierce BCA Protein Assay Reagent A
Formulation Bicinchonic acid and tartrate in an alkaline carbonate buffer
Quantity 1L
Sufficient For 5000 microplate assays when mixed with Reagent B

Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
 
Highlights:
  • Colorimetric – estimate visually or measure with a standard spectrophotometer or plate reader (562nm)
  • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
  • Compatible – unaffected by typical concentrations of most ionic and nonionic detergents
  • Moderately fast – much easier and four times faster than the classical Lowry method
  • High linearity – linear working range for BSA equals 20 to 2000µg/mL
  • Sensitive – detect down to 5µg/mL with the enhanced protocol
BCA Protein Assay Applications:
  • Studying protein:protein interactions
  • Measuring column fractions after affinity chromatography
  • Estimating percent recovery of membrane proteins from cell extracts
  • High-throughput screening of fusion proteins

Alternative Products
Documentation

Technical documents