Thermo Scientific™ Pierce™ BCA™ Protein Assay

Green-to-blue (A562nm), two-component, precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 23227

1 SET BCA PROTEIN ASSAY KIT, 500ML KIT

UNSPSC: 41116012

Code: PN

Additional Details:
Additional Details: CAS Number: 7758-99-8 Weight: 1.90000kg Transport: UN number : 3082 Chem class : 1 Pack group : III



Product Code. 10678484

Quantity Price
1 £ 128.0 / 500mL
EU Stock 364
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Description and Specification

Specification

Description Pierce BCA Protein Assay Kit
Formulation Three-component kit with BCA reagents and standard
Kit Contents Albumin Standard Ampules, 2mg/mL, 10 x 1mL
Quantity 500mL kit
Sufficient For 250 tube assays or 2500 microplate assays

Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
 
Highlights:
  • Colorimetric – estimate visually or measure with a standard spectrophotometer or plate reader (562nm)
  • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
  • Compatible – unaffected by typical concentrations of most ionic and nonionic detergents
  • Moderately fast – much easier and four times faster than the classical Lowry method
  • High linearity – linear working range for BSA equals 20 to 2000µg/mL
  • Sensitive – detect down to 5µg/mL with the enhanced protocol
BCA Protein Assay Applications:
  • Studying protein:protein interactions
  • Measuring column fractions after affinity chromatography
  • Estimating percent recovery of membrane proteins from cell extracts
  • High-throughput screening of fusion proteins

Documentation

Technical documents