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Thermo Scientific™ Pierce™ BCA™ Protein Assay

Green-to-blue (A562nm), two-component, precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Brand:  Thermo Scientific™ 23227

Code : PN

Additional Details : CAS Number : 7758-99-8 Weight : 1.90000kg Transport : UN number : 3082 Chem class : 1 Pack group : III


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Product Code. 10678484

  • £128.00 / 500mL


EU Stock 209
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Description

Description

Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
 
Highlights:
  • Colorimetric – estimate visually or measure with a standard spectrophotometer or plate reader (562nm)
  • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
  • Compatible – unaffected by typical concentrations of most ionic and nonionic detergents
  • Moderately fast – much easier and four times faster than the classical Lowry method
  • High linearity – linear working range for BSA equals 20 to 2000µg/mL
  • Sensitive – detect down to 5µg/mL with the enhanced protocol
BCA Protein Assay Applications:
  • Studying protein:protein interactions
  • Measuring column fractions after affinity chromatography
  • Estimating percent recovery of membrane proteins from cell extracts
  • High-throughput screening of fusion proteins
Specifications

Specifications

Pierce BCA Protein Assay Kit
Albumin Standard Ampules, 2mg/mL, 10 x 1mL
250 tube assays or 2500 microplate assays
Three-component kit with BCA reagents and standard
500mL kit
Safety and Handling

Safety and Handling

Product Identifier
  • SET BCA PROTEIN ASSAY KIT, 500ML KIT

Signal Word
  • Warning

Hazard Category
  • LONG-TERM AQUATIC HAZARD Chronic 2
  • ACUTE AQUATIC Acute 1

Hazard Statement
  • H400-Very toxic to aquatic life.
  • H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement
  • P273-Avoid release to the environment.
  • P391-Collect spillage.
  • P501-Dispose of contents/container to A§.

Documentation

Technical Documents