Avidin Protein and Conjugates, Invitrogen™

Perform Western blot, ELISA or other biotin-based detection methods using these purified and HRP-, AP- or FITC-labeled avidin molecules.

Overview
Brand: Thermo Scientific Pierce

Manufacturer Part Number: 21121

AVIDIN 10MG

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: CAS Number: 500-22-1 Weight: 1.81440kg



Product Code. 11836814

Quantity Price
1 £ 87.5 / 10mg
EU Stock 6
Add to basket


Description and Specification

Specification

Description Pierce Avidin
Formulation Lyophilized powder from protein in salt-free solution
Quantity 10mg
Sufficient For Binding 11 to 15ug biotin per mg protein

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.

Highlights:

  • Native avidin – glycoprotein from chicken egg whites, 67kDa, pI equals 10

  • Soluble – glycosylation and isoelectric point renders avidin more soluble than streptavidin

  • Affordable – significantly less expensive than recombinant streptavidin

  • ABC staining – avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)

  • Pure protein and conjugates – choose pure, unconjugated avidin for use in ABC staining or peroxidase or alkaline phosphatase forms for membrane and plate-based assays

Documentation

Technical documents