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Thermo Scientific™ Pierce™ Amino Acids for SILAC Labeling

Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Brand:  Thermo Scientific™ 88209

Code : PN

Additional Details : CAS Number : 189281-66-1 Weight : 0.80000kg

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Product Code. 11874121

  • £316.00 / 50mg


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Description

Description

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy“ 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Efficient – 100% label incorporation into proteins of living cells

  • Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases

  • Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment

  • High-quality supplements – heavy amino acids with >99% isotope purity

Specifications

Specifications

L-Lysine-2HCl, 13C6, 15N2
SILAC experiments (stable isotope labeling with amino acids in cell culture)
Media for one SILAC experiment
Lyophilized powder of L-Lysine-2HCl with at least 99% 13C6, 15N2
50mg
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