Thermo Scientific™ Pierce™ Abundant Protein Depletion Spin Columns

Decrease the abundant albumin and antibody components of human plasma samples in preparation for mass spectrometry or 1D and 2D gel electrophoresis.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 85164

X6 Top 12 Abundant Protein Depletion spin columns

UNSPSC: 41115404

Code: PN

Additional Details:
Additional Details: Weight: 1.36080kg



Product Code. 13424319

Quantity Price
1 £ 206.0 / Pack of 6
EU Stock 3
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Description and Specification

Specification

Description Pierce Top 12 Abundant Protein Depletion Spin Columns
Formulation Microcentrifuge spin columns containing 0.81mL of affinity resin, supplied as a 62% slurry in PBS buffer (pH 7.4) with sodium azide
Quantity 6 columns
Sufficient For Removing 12 proteins from 6 human serum samples, 10┬ÁL each

The Pierce Top 2 Abundant Protein Depletion Spin Columns use highly specific immobilized anti-HSA and anti-IgG antibodies to remove human serum albumin (HSA) and all major subclasses of gamma globulin (IgG) from serum, plasma or spinal fluids. Similarly, the Pierce Top 12 Abundant Protein Depletion Spin Columns are designed to remove HSA, IgG and 10 other high-abundance proteins from human serum or plasma. The resins are provided in an economical and convenient spin column specifically designed one step processing and for single use.

Highlights:

  • Optimized – resin in spin columns is scaled and optimized for treating 10μL (600μg) of human plasma samples for mass spectrometry and/or 1D and 2D electrophoresis

  • Efficient – kits remove >90% of IgG and >95% of albumin, plus other abundant proteins (Top 12)

  • Fast – process samples in 40 to 60 minutes (depending on resin)

  • Economical – cost effective spin columns are priced for single use

  • Reduce variability – One time use prevents abundant protein carryover and experimental variability

  • Two resin options – choose the system appropriate for your need: 2 or 12 protein depletion columns

Top 2 Spin Columns Remove:

  • Albumin
  • IgG

Top 12 Spin Columns Remove:

  • Albumin
  • IgG
  • α1-Acid Glycoprotein
  • α1-Antitrypsin
  • α2-Macroglobulin
  • Apolipoprotein A-I
  • Apolipoprotein A-II
  • Fibrinogen
  • Haptoglobin
  • IgA
  • IgM
  • Transferrin