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Thermo Scientific™ Phusion™ Hot Start II High-Fidelity DNA Polymerase

Optimize PCR with one of the most accurate hot start DNA polymerases on the market, featuring extreme specificity and improved performance.

£96.75 - £443.00

Specifications

Description Phusion™ Hot Start II High-Fidelity DNA Polymerase
For Use With (Application) Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
Includes 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration
Storage Requirements Recommended storage temperature -20°C. Stable for one year from the assay date.
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10628439 Thermo Scientific™
F549L
500U (2U/µL) £443.00
500 units
EU Stock 100
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10441338 Thermo Scientific™
F549S
100U (2U/µL) £96.75
100 units
EU Stock 78
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Description

Description

Highlights

  • Reaction set up can be done at room temperature
  • Reduces non-specific amplification
  • Prevents primer degradation during set up
  • Zero-time reactivation due to unique hot start technology
  • Extreme fidelity (52X Taq)
  • Speed of the polymerase allows short extension times
  • Robust reactions, minimal optimization needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with Green Buffer
Specifications

Specifications

Phusion™ Hot Start II High-Fidelity DNA Polymerase
Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration
Recommended storage temperature -20°C. Stable for one year from the assay date.

Current Phusion Hot Start customers can switch to Phusion Hot Start II with no changes needed to existing protocols or primers. Original Phusion Hot Start will remain available for purchase, however, all new customers are advised to purchase Phusion Hot Start II to allow more flexibility in primer design.