Thermo Scientific™ Phusion Hot Start High-Fidelity DNA Polymerase

Increase the specificity of PCR amplification by preventing non-specific extension with this highly accurate DNA polymerase, now available with a hot start modification.

Brand: Thermo Scientific™

Manufacturer Part Number: F-540S

UNSPSC: 41106314

Code: 87

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 11571405

Quantity Price
1 £ 107.0 / 100 units
EU Stock 8
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  • Description and Specification
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Description and Specification


For Use With (Application) Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
Storage Requirements Recommended storage temperature -20°C. Stable for one year from the assay date.
Quantity 100U (2U/µL)
Includes Phusion Hot Start High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration.
Description Phusion™ Hot Start High-Fidelity DNA Polymerase

Storage buffer

20 mM Tris-HCl (pH 7.4 at 25°C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 µg/ml BSA and 50% glycerol.


Recommended storage temperature -20°C.

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